Table 2.
Oligonucleotides used in this study.
| Primer | Sequence 5′−3′ | Restriction site | Purpose |
|---|---|---|---|
| Ari1-F | TCGTTCGAAAAAATGGCGACTACTGATACCACTGTTTTCGTTTCTG | BstBI | ari1 gene amplification |
| Ari1-R | TCGCTCGAGTTAGTGATGGTGATGGTGATGGGCTTCATTTTGAACTTC | XhoI | |
| VTF | TTCGAAACGATGGGTACTAC | — | Verification of tps1 or ari1 gene insertion |
| VAF | TTCGAAAAAATGGGTACTAC | — | |
| VR | AGTGATGGTGATGGTGATGG | — | |
| qTps1-F | TTGTGGTGTCCAACAGGCTT | — | quantitative real-time PCR |
| qTps1-R | GGACGACATTGCGTACTCGT | — | |
| qAri1-F | TTGTGCTACACACTGCCTCC | — | |
| qAri1-R | CGTTCACTGCAGGGGTTAGT | — | |
| qTaf10-F | TCCAGGATCAGGTCTTCCGT | — | |
| qTaf10-R | TGTCCTTGCAATAGCTGCCT | — |
The underlined bases encode restriction site and italicized bases encode 6xHis-tag.