Table 3.
Bacterial strains and plasmids used in this study.
| Strains and plasmids | Relevant properties | Source or reference |
|---|---|---|
| Strains | ||
| Escherichia coli DH5α | F−,φ80dlacZ△M15,△(lacZYA-argF)U169, deoR, recA1, endA1, hsdR17(rk-,mk+) | Invitrogen |
| Escherichia coli BL21(DE3) | F−, ompT, hsdSB (rB−mB−), ga, dcm, λ(DE3) | Invitrogen |
| Q1463 | △prpR::lacI PT7gdrAB PT7dhaB123 | Ref. 14 |
| Q1638 | Q1463 carrying pWQ02 | Ref. 14 |
| Q2537 | Escherichia coli DH5α carrying pY01 | This study |
| Q2549 | Escherichia coli DH5α carrying pY02 | This study |
| Q2572 | Q1638 carrying pY03 | This study |
| Q2582 | Escherichia coli DH5α carrying pY03 | This study |
| Q2588 | Q1638 carrying pY02 | This study |
| Q2646 | Q1463 carrying pY05 | This study |
| Q2647 | Q1463 carrying pY04 | This study |
| Plasmids | ||
| pBAD18 | KmR, cloning vector, pBR322 origin, araBAD promotor | NBRP-E. coli at NIG |
| pET21a | AmpR, pBR322 origin, lacI PT7 | Novagen |
| pACYCDuet-1 | CmR, p15A origin, lacI PT7 | Novagen |
| pWQ02 | AmpR, pET21a carrying polyhydroxyalkanoate synthase phaC and propionaldehyde dehydrogenase pduP | Ref. 15 |
| pWQ04 | CmR, pACYCDuet-1 carrying glycerol dehydratase dhaB123 and its reactivating factor gdrAB | Ref. 15 |
| pY01 | KmR, pBAD18 carrying lysis genes S, R, and Rz | This study |
| pY02 | KmR, pBAD18 carrying lysis genes S, R, and Rz and promoter PmgtA | This study |
| pY03 | KmR, pBAD18 carrying lysis genes S, R, and Rz, and promoter PmgtA-UTR | This study |
| pY04 | AmpR, pET21a carrying phaC, pduP, lysis genes S, R, and Rz, and promoter PmgtA | This study |
| pY05 | AmpR, pET21a carrying phaC, pduP, lysis genes S, R, and Rz, and promoter PmgtA-UTR | This study |