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. 2017 Jan 25;8(5):488–500. doi: 10.1080/21655979.2017.1284712

Figure 5.

Figure 5.

Western blot analysis of E. coli C41 expressing INP and harboring E-lysis plasmid pGLysivb or pGLMivb, respectively, and their derived BGs. Samples were taken at time point of E-lysis induction (Tp - 0 min) and after β-propiolactone treatment of BG samples. Western blotting was performed with rabbit anti-H-NINP serum and anti-rabbit IgG horseradish peroxidase conjugated antibodies. Lane1: E. coli C41 (pBINP, pGLMivb); lane 2: INP-BG-pLM, BG derived form of E. coli C41 (pBINP, pGLMivb); lane 3: E. coli C41 (pBH-NINPL, pGLysivb); lane 4: INP-BG-pLy, BG derived form of E. coli C41 (pBINP, pGLysivb); lane 5: E. coli C41 (pBAD, pGLysivb); lane 6: BG, BG derived form of E. coli C41 (pBAD, pGLysivb). Positions of molecular size marker proteins are indicated in kilodaltons (kDa).