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. 2017 Feb 17;8(5):600–612. doi: 10.1080/21655979.2017.1292186

Table 2.

Summary of purification step for recombinant human HSA- eTGFBR2.

Step Total protein(mg) HSA-eTGFBR2(mg) Recovery rate(%) Purity(%)
Crude supernatants 500 180 100 36
Blue Sepharose Chromatography 200 123 68 61.5
Q Sepharose Chromatography 77 70 38.9 >90

Estimated by BCA protein assay, ELISA and coomassie blue-stained SDS–PAGE gel.