Table 2.
Step | Total protein(mg) | HSA-eTGFBR2(mg) | Recovery rate(%) | Purity(%) |
---|---|---|---|---|
Crude supernatants | 500 | 180 | 100 | 36 |
Blue Sepharose Chromatography | 200 | 123 | 68 | 61.5 |
Q Sepharose Chromatography | 77 | 70 | 38.9 | >90 |
Estimated by BCA protein assay, ELISA and coomassie blue-stained SDS–PAGE gel.