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. Author manuscript; available in PMC: 2017 Oct 13.
Published in final edited form as: Cell Rep. 2017 Sep 26;20(13):3123–3134. doi: 10.1016/j.celrep.2017.09.010

Figure 2. The WW domain of Pasha associates with the phosphorylated CTD of Pol II.

Figure 2

See also Figure S1. (A) Scheme of assay for binding between GST proteins and phospho-Pol II present in nuclear extracts. (B) Nuclear extract was incubated with GST proteins, as indicated, and pulled down material was probed for S2-phosphorylated Pol II. Input represents 4% of the total pulldown reaction. (C) Binding between GST proteins and biotinylated peptides containing four CTD heptad repeats. Peptide-associated GST proteins were visualized by Western blot. Inputs shown represent 5% of total binding reactions. (D) Biotinylated peptides with different phospho-modifications were assayed for binding to GST-WWD. Input is 5% of the binding reactions.