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. 2017 Aug 18;13(10):1664–1678. doi: 10.1080/15548627.2017.1345411

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© 2017 The Author(s). Published with license by Taylor & Francis

This is an Open Access article distributed under the terms of the Creative Commons Attribution-NonCommercial-NoDerivatives License (http://creativecommons.org/licenses/by-nc-nd/4.0/), which permits non-commercial re-use, distribution, and reproduction in any medium, provided the original work is properly cited, and is not altered, transformed, or built upon in any way.

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Figure 4. — The DHA-mediated inflammatory tolerance is related to the formation of SQSTM1/p62-bodies. (A) IB analysis of MDMs treated with DHA (70 μM) for the indicated times followed by LPS for 1 h. The quantification (below) represents mean fold changes ± SEM of DHA + LPS compared to vehicle + LPS on a log scale, n = 6, (Friedman test with Dunn's). (B) QRT-PCR analysis of CXCL10 levels in MDM after 16 h DHA with and without addition of 5 mM NAC followed by 3 h LPS, n = 3, (repeated-measures ANOVA with Dunnett's). (C) QRT-PCR analysis of CXCL10 in SQSTM1 siRNA-transfected MDMs treated with DHA for 16 h and LPS for 3 h, n = 5, (Friedman test with Dunn's). (D) IB analysis of IRF3, RELA and IKBKB phosphorylation in control and SQSTM1 knockdown MDMs, n = 6 (Friedman test with Dunn's). (E) CXCL10 protein levels in Sqstm1^+/+ (n = 8) and sqstm1⁻^/⁻ (n = 10) male mice were measured by ELISA. Serum was analyzed at baseline and at 3 and 6 h after challenge with 3.5 µg/g LPS. ND, not detected.