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. 2017 Aug 18;13(10):1664–1678. doi: 10.1080/15548627.2017.1345411

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© 2017 The Author(s). Published with license by Taylor & Francis

This is an Open Access article distributed under the terms of the Creative Commons Attribution-NonCommercial-NoDerivatives License (http://creativecommons.org/licenses/by-nc-nd/4.0/), which permits non-commercial re-use, distribution, and reproduction in any medium, provided the original work is properly cited, and is not altered, transformed, or built upon in any way.

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Figure 5. — KEAP1 is sequestered in DHA-induced SQSTM1/p62-bodies and contributes to inflammatory tolerance. (A) IP of SQSTM1 from MDMs treated with DHA for 16 h and IB analysis of KEAP1 and TAX1BP1; INPUT (10%). Data are representative of 3 independent experiments. (B) Immunostaining for SQSTM1 and KEAP1 in MDMs treated with DHA for 24 h (scale bars: 10 µm). (C) IB analysis and quantification (below) of SQSTM1 and KEAP1 in soluble and insoluble fractions from RAW264.7 cells after 16 h DHA, n = 4 (repeated measures ANOVA). (D) QRT-PCR analysis of CXCL10 or (E) CXCL11 in KEAP1 siRNA-transfected MDMs treated with DHA for 16 h and LPS for 3 h, n = 5 (Friedman test with Dunn's). (right) Comparison of the downregulating effect of DHA in control or KEAP1 siRNA-treated MDMs from (D) or (E) respectively, n = 5 (Mann Whitney test).