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. 2017 Oct 13;12(10):e0186128. doi: 10.1371/journal.pone.0186128

Fig 5. Severe burn injury activates UPS and autophagy, and the former may be inhibited by 4-PBA treatment to some extent.

Fig 5

The rats were randomized to four groups: sham, sham+4-PBA, burn and burn+4-PBA. Markers of the muscle-specific ubiquitin-proteasome pathway (including atrogin-1 and MuRF1) and of autophagy (LC3-II/LC3-I ratio) were assessed in tibialis anterior muscle (TAM) specimens post-burn by western blot. Representative blots and statistical histograms showed the changes in various marker proteins at different time points after burn injury (A) and the relieving effects of 4-PBA at 7 days post-burn (B). The amount of proteins was quantified as normalized to the internal control GAPDH. n = 6 per group. *p<0.05, ** p<0.01 vs. sham group; # p<0.05 vs. burn group.