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. 2017 Oct 13;12(10):e0186423. doi: 10.1371/journal.pone.0186423

Fig 2. HCE is the target of H2O2.

Fig 2

All PICs were treated with 0.6 mM H2O2 for 10 min, then quenched with 6 mM DTT. Indicated addbacks occurred after the quench and 5 min before the pulse. A, Addback of either HeLa nuclear extract (HNE) or a Mono Q fraction of HNE. Bottom panel shows Western blot for HCE in indicated fractions. B, Addback of HNE, Mono Q fraction 13 or indicated amounts of HCE (pmol).