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. 2017 Oct 13;13(10):e1006668. doi: 10.1371/journal.ppat.1006668

Fig 4. ST binds TRRAP and MYCL in absence of EP400.

Fig 4

(A) MKL-1 cells containing three different Dox-inducible shRNA targeting EP400 (shEP400–1, shEP400-2, or shEP400-3) or shScramble (shScr) treated with Dox (0.3 μg/ml) every two days for five days. Lysates (Input) were immunoprecipitated with EP400 or control IgG antibodies and blotted for cells before (-) or after (+) 5 days of Dox treatment. (B) Same as 4A except lysates were immunoprecipitated with control IgG, MAX or Ab5 antibodies and blotted for cells after (+) 5 days of Dox treatment. (C) Cell viability assay of MCPyV positive MCC cell line MKL-1 containing Dox-inducible shRNA targeting EP400 (shEP400) or scramble (shScr). Dox added for indicated number of days. Three biological replicas were performed. Data are presented as mean (SD). (D) Lysates from UISO cells containing an inducible scramble shRNA (shScr) or 3 different shRNAs specific for EP400, prepared after 5 days Dox treatment were immunoblotted (Input) or immunoprecipitated with EP400 antibody or control IgG and blotted with indicated antibodies. (E) Cell viability assay of MCPyV negative MCC cell line UISO containing Dox-inducible shRNA targeting EP400 (shEP400) or scramble (shScr). Dox added for indicated number of days. Three biological replicas were performed; data are presented as mean (SD). (F) Lysates from parental Kelly cells or containing Dox inducible scramble shScr or shEP400-1 prepared after 5 days Dox treatment were immunoblotted (Input) or immunoprecipitated with MAX antibody or non-specific IgG and blotted with antibodies indicated. (G) Cell viability assay of Kelly cells containing Dox-inducible shRNA targeting EP400 (shEP400) or scramble (shScr). Three biological replicas were performed; data are presented as mean (SD).