Fig 5. Effect of Ascaris suum infection and grape pomace supplementation on mucosal granulocyte numbers and chemokine expression.

Numbers of eosinophils in the duodenum, jejunum and ileum (A), mast cells in the jejunum (B) and gene expression of three different chemokines in the jejunal mucosa (C) of pigs fed either a control diet (black) or a diet supplemented with grape pomace (red), and either infected for 14 days with Ascaris suum or not infected. Shown is mean ± SD ***P<0,005, *P<0.05 by two-way ANOVA with Bonferroni post-hoc testing. Gene expression data is relative expression of each gene normalized to the reference genes B2M, RPL13A and TBP. All cell counts are expressed in cells / mm² tissue.