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. 2017 Oct 6;10:4895–4904. doi: 10.2147/OTT.S141008

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© 2017 Li and Zhao. This work is published and licensed by Dove Medical Press Limited

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AEG-1 is a functional target of let-7d.

Notes: (A) The putative let-7d binding site in the 3′ UTR of AEG-1 is shown. (B) The relative luciferase activity was determined by luciferase reporter assay in IOMM-Lee and CH-157MN cells co-transfected with AEG-1-wt or AEG-1-mut and let-7d mimic or miR-control. (C and D) qRT-PCR and Western blot analyses revealed the mRNA and protein levels of AEG-1 in IOMM-Lee and CH-157MN cells transfected with let-7d mimics or inhibitor. ^*P<0.05 vs controls.

Abbreviations: AEG-1, astrocyte elevated gene-1; mut, mutation; NC, normal control; UTR, untranslated region; qRT-PCR, quantitative real-time polymerase chain reaction; wt, wild type.