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. Author manuscript; available in PMC: 2019 Jan 1.
Published in final edited form as: Biochim Biophys Acta. 2017 Apr 13;1860(1):83–90. doi: 10.1016/j.bbamem.2017.04.008

Figure 2.

Figure 2

Rapid changes in Cx43 phosphorylation occur in response to compounds that induce gap junction disassembly. Immunoblot analysis of NRK lysates with antibody to total Cx43 shows loss of the P0 form of Cx43 within 5 minutes of treatment with EGF 20ng/ml or TPA 50nM (Sigma). Linescans of the band densities are shown on the right. Antibodies and methods used are as previously described [104].