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. Author manuscript; available in PMC: 2019 Jan 1.
Published in final edited form as: Biochim Biophys Acta. 2017 Apr 13;1860(1):83–90. doi: 10.1016/j.bbamem.2017.04.008

Figure 3.

Figure 3

Spatiotemporal changes in gap junction size and phosphorylation during induced disassembly. NRK cells were fixed and stained with a mouse antibody to total Cx43 (green) and a rabbit antibody to Cx43 phosphorylated on S373 (red). Gap junctions in control cells range in size and show little signal for pS373. After 5 minutes of TPA treatment (100nM) gap junction size is increased and Cx43 is phosphorylated on S373 (yellow). After 30 minutes of treatment gap junctions are diminished, disorganized and in various states of internalization. Antibodies and methods used were as described in [53]. (bar=25μm).