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. 2017 Oct 5;68(1):210–223.e6. doi: 10.1016/j.molcel.2017.09.012

Figure 5.

Figure 5

Rigosertib Inhibits Microtubule Growth In Vitro

(A) Kymographs illustrating in vitro dynamics of microtubules grown in the presence of 15 μM tubulin in the absence (control) or presence of 5, 10, or 20 μM rigosertib. Rhodamine-labeled-tubulin (0.5 μM) was added in the reaction to visualize the microtubules.

(B and C) Quantification of microtubule growth rate (B) and catastrophe frequency (C) with 15 μM tubulin alone or with 5, 10, or 20 μM rigosertib as illustrated in (A). n = 102 for control; n = 102, 137, and 110 for 5, 10, and 20 μM rigosertib, respectively.

(D) Kymographs of fluorescently labeled EB3 illustrating in vitro dynamics of microtubules grown in the presence of 15 μM tubulin along with EB3 (20 nM) and in the absence (control) or presence of 1, 5, or 10 μM rigosertib.

(E and F) Quantification of microtubule growth rate (E) and catastrophe frequency (F) with 15 μM tubulin along with EB3 (20 nM) without or with 1, 5, or 10 μM rigosertib as illustrated in (D). n = 75 for control; n = 102, 110, and 82 for 1, 5, and 10 μM rigosertib, respectively.

Data are presented as mean ± SEM.