REAGENT or RESOURCE	SOURCE	IDENTIFIER
Antibodies
Mouse monoclonal anti-α-Tubulin (clone DM1A)	Sigma-Aldrich	Cat#T9026; RRID: AB_477593
Chemicals, Peptides, and Recombinant Proteins
Rigosertib	Selleck Chemicals	Cat#S1362
Deposited Data
Cell imaging data	This paper, Mendeley Data	https://doi.org/10.17632/dfdxy4ksh3.1
In vitro microtubule polymerization assay imaging data, no EB3 (Figure 5A)	This paper, Mendeley Data	https://doi.org/10.17632/tm22dtht9x.1
In vitro microtubule polymerization assay imaging data, with EB3 (Figure 5D)	This paper, Mendeley Data	https://doi.org/10.17632/t6g8bfpgvk.1
Tubulin:rigosertib crystal structure	This paper	PDB: 5OV7
Rigosertib sensitivity and untreated growth phenotypes from genome-wide and focused sublibrary screens	This paper	Data S1
Experimental Models: Cell Lines
K562 CRISPRi	Gilbert et al., 2014	N/A
K562 CRISPRa	Gilbert et al., 2014	N/A
cMJ009 (K562 constitutive CRISPRi with rtTA)	This paper	N/A
RPE1 CRISPRi	This paper	N/A
RPE1 CRISPRa	This paper	N/A
Oligonucleotides
See Table S1 for protospacer sequences	This paper	N/A
Recombinant DNA
CRISPRi-v1 library	Gilbert et al., 2014	Addgene Cat#62217
CRISPRa-v1 library	Gilbert et al., 2014	Addgene Cat#60956
hCRISPRi-v2 rigosertib sublibrary	This paper	N/A
hCRISPRa-v2 rigosertib sublibrary	This paper	N/A
pU6-sgRNA EF1alpha-puro-T2A-BFP	Gilbert et al., 2014	Addgene Cat#60955
pU6-sgRNA EF1alpha-puro-T2A-GFP	Gilbert et al., 2014	N/A
pCRISPRia-v2	Horlbeck et al., 2016	Addgene Cat#84832
Software and Algorithms
ScreenProcessing pipeline	Horlbeck et al., 2016	https://github.com/mhorlbeck/ScreenProcessing
Micro-Manager microscope control software	Edelstein et al., 2014	https://micro-manager.org/