Fig. 3.

Loss of Ccdc88b on inflammatory response to DSS-induced colitis. WT and Ccdc88b ^mut mice were treated with 3% DSS for 5 days followed by 3 days of water. a Relative expression levels of indicated cytokine and chemokine mRNAs extracted from WT and Ccdc88b ^mut colon mice at day 4 (n = 5) and day 8 (n = 10) following DSS treatment (mean±SEM; *P < 0.05, **P < 0.01; two-tailed Student’s t-test). b Proinflammatory cytokine MCP1 and KC were quantified in serum by ELISA, and results are presented in ng/ml (mean ± SEM; ***P < 0.001; two-tailed Student’s t-test). a, b Data are pooled from 2 independent experiments. c Representative image (n = 4) of crypt regeneration visualized by Ki-67 staining (hematoxylin counterstaining), Scale bars, 100 µm. d Representative image (n = 4) of epithelial integrity by mucin staining (Alcian blue) of colons from DSS-treated mice at day 8. Scale bars, 100 µm