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. 2017 Oct 13;7:13167. doi: 10.1038/s41598-017-13451-8

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© The Author(s) 2017

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Groups of amino acids (AA) and metabolites that differ significantly between control (CNT), E2 and Testos. (A) branched-chain AA (BCAA), (B) ketogenic AA, (C) aromatic AA (AAA), (D) glucogenic AA, (E) glutaminolysis AA, (F) L-Glutamic acid/L-Glutamine ratio, (G) total AA, (H) total acylcanitines (AC), (I) total sphingomyelins (SM), (J) total acyl-alkyl phosphatidylcholines (PC ae), (K) total diacyl phosphatidylcholines (PC aa), and (L) total lyso-phosphatidylcholines (LPC). Asterisks denote significant differences from control.