Fig. 6.

AR regulates expression of miR-185-5p that targets HIF2α and VEGF-C. a Mutations introduced at the target sequence of miR-185-5p in HIF2α and 3′ UTR of VEGF-C. b, c Luciferase reporter assays showing miR-185-5p can target HIF2α promoter (b) and VEGF-C 3′ UTR (c) in SW839 cells. d Bioinformatics analysis of potential AR binding sites on the miR-185-5p promoter using online software ALGGEN PROMO. e ChIp assay showing AR can bind to several potential binding sites on the miR-185-5p promoter. f Promoter truncation showing that AR binding to region between −1527 to −1520 and −969 to −818 bases of miR-185-5p promoter is important for AR’s regulation of miR-185-5p. Activity of 5K promoter (−5000 to −74) in SW839-control was used as a control. All experiments were carried out in triplicate. *p < 0.05, ***p < 0.001 were considered statistically significant by t-test for two groups or ANOVA for more than two groups