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. 2017 Oct 13;7:13113. doi: 10.1038/s41598-017-13302-6

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© The Author(s) 2017

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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ApoAI and lipoprotein receptors were co-localized with lipid A in blood-brain interfaces. Double immunofluorescence of Lipid A and ApoAI, SR-BI, ApoER2 or LDLr were made in Medulla oblongata (A–D), lateral ventricle (E–H), septal vein (I–L) and subfornical organ (M–P). In all cases green corresponds to Lipid A immunosignal and blue DAPI staining in nucleus. Red immunosignal corresponds to ApoAI (A, E, I and M), SR-BI (B,F,J and N), ApoER2 (C,G,K, and O) and LDLr (D,H,L and P). Arrows indicate green and red immunosignals overlapping in astrocyte-like cells (A–D), tanycyte-like cells from the lateral ventricle (E–H), endothelial-like cells (I–L) and ependymal cells from the subfornical organ (M–P). Head arrows indicate green and red immunosignals overlapping in meninges. Corpus callosum (cc), choroid plexus (chp), hippocampal fissure (fi), ventral hippocampal commissure (vhc), subfornical organ (SFO) and septal vein (sv). Scale bars = 20 µm.