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. 2017 Oct 13;7:13113. doi: 10.1038/s41598-017-13302-6

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© The Author(s) 2017

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Astrocytes and endothelial cells co-localized Lipid A with ApoAI and lipoprotein receptors. Triple immunofluoresences of Lipid A, GFAP and ApoAI, SR-BI or ApoER2; and Lipid A RECA-1 and ApoAI, SR-BI or ApoER2 were made in Medulla oblongata (A–C) and septal vein (D–F). In all cases, green corresponds to Lipid A. Red corresponds to GFAP (A–C), ApoAI (D), SR-BI (E) and ApoER2 (F) immunosignals. Magenta corresponds to ApoAI (A), SR-BI (B) and ApoER2 (C) immunosignals. Blue corresponds to RECA-1 (D–F) immunosignal. Arrows indicate green, red and magenta immunosignals overlapping in astrocytes (A–C), and green, red and blue immunosignals overlapping in endothelial cells (D–F). Head arrows indicate green, red and magenta immunosignals overlapping in meninges (A–C). Septal vein (SV). Ventral hippocampal commissure (vhc). Scale bars = 20 µm.