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. 2017 Oct 13;7:13113. doi: 10.1038/s41598-017-13302-6

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© The Author(s) 2017

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Specific antibodies against lipid A and Core LPS regions can detect the LPS in brain tissue homogenates. (A) Western blot of lipid A LPS region in medulla oblongata, frontal cortex (FC), liver and distal colon (DC). (B) Western blot of Core LPS region in medulla oblongata, frontal cortex (FC), liver and distal colon (DC). Purified E. coli LPS (E. coli LPS), Control distal colon (ctrl DC), medulla oblongata (medulla), frontal cortex (PFC), Antibiotic decontamination treatment and chronic mild stress condition distal colon (ATB/ CMS DC), chronic mild stress condition distal colon (CMS DC).