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. 2017 Oct 13;8:928. doi: 10.1038/s41467-017-00988-5

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© The Author(s) 2017

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/..

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Fig. 6 — Nt-acetylation of histone H4 antagonizes phosphorylation of histone H4 serine 1 to regulate Slug expression. a Western blot analysis of indicated histone H4 modifications in Scr and NatD-KD H1299 cells. Histone H4 served as a loading control. b ChIP analysis of the enrichment of indicated histone H4 modifications on the Slug promoter in Scr and NatD-KD H1299 cells. IgG served as a negative control. Results are shown as mean ± s.d. from three independent experiments; two-tailed Student’s t-test, *P < 0.05, **P < 0.01 compared with the Scr control. c ChIP analysis of the enrichment of H3K4me3 and H3K27me3 on the Slug promoter in Scr and NatD-KD H1299 cells. IgG served as a negative control. Results are shown as mean ± s.d. from three independent experiments; two-tailed Student’s t-test, **P < 0.01 compared with the Scr control. d Western blot analysis of indicated proteins from H1299 cells overexpressing NatDΔ or wild-type NatD. Histone H4 served as a loading control. e ChIP analysis of the enrichment of Nt-ac-H4 and H4S1ph on Slug promoter in H1299 cells overexpressing NatDΔ or wild-type NatD. IgG served as a negative control. Results are shown as mean ± s.d. from three independent experiments; two-tailed Student’s t-test, *P < 0.05, **P < 0.01 compared with the wild-type control