Table 1.

Effect of ionic strength on kinetic and equilibrium parameters of the interaction between wheat ATI and TLR4.

Ionic strength conditions	kass (M−1s−1)	kdiss (s−1)	KD (M)
20 mM PBS (no NaCl)	43000 ± 2000	0.0001 ± 0.00005	(2.3 ± 1.2) × 10−9
20 mM PBS (25 mM NaCl)	39000 ± 4000	0.0004 ± 0.0002	(1.0 ± 0.5) × 10−8
20 mM PBS (50 mM NaCl)	40000 ± 2500	0.0007 ± 0.0001	(1.8 ± 0.3) × 10−8
20 mM PBS (140 mM NaCl)	41000 ± 6000	0.0025 ± 0.0006	(6.1 ± 1.7) × 10−8