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. 2017 Oct 13;7:13138. doi: 10.1038/s41598-017-13744-y

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© The Author(s) 2017

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Cell metabolic activity on rat cardiomyoblasts H9C2 cells, after 24 hours and confocal imaging after microspheres internalization. alamarBlue® assay was carried out on: (A) the synthesized polypropylene sulfide (PPS) in orange, PEG2000 (blue), untreated cells (green); (B) collagen-PPS hollow spheres (light blue), polystyrene (green) and untreated cells (orange) were used as controls (n=3, p < 0.05). Confocal images of H9C2 rat cardiomyoblasts incubated for 24 hours with FITC-labeled collagen-PPS hollow spheres (green) at 50 μg/mL (C); and 100 μg/mL (D). Nuclei were stained with DAPI (blue), while actin fibers were stained with rhodamine phalloidin (red).