Table 2.

Clopper-Pearson 95% CI for prevalence of Bsal and Bd in captive pet collections.

Chytrid Fungus	Validated Number of Samples Tested	Number Positive	Proportion Infected	Clopper-Pearson 95% Confidence Interval	Probability of detecting at least one positive individual (assuming prevalence = 0.01)
Batrachochytrium salamandrivorans	537	0	0.0000	0.0000–0.0071	0.9955
Batrachochytrium dendrobatidis	537	7	0.0130	0.0053–0.0267	0.9955

This suggests that both Bsal, if present, and Bd are at low prevalence in pet collections in the U.S. Probability of detecting at least one positive individual assuming prevalence was p = 0.01 was calculated with the following equation D = 1-(1-p)ⁿ after incorporating our confidence in DNA quality (see Methods).