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. 2017 Oct 13;7:13168. doi: 10.1038/s41598-017-13205-6

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© The Author(s) 2017

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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(a) 3D plot obtained with image processing showing the cross-correlation matrix value (mesh grid) overlaid with the fitted Gaussian function (color). The peak position of the Gaussian function is used to determine the bead displacement. Inset: A typical bright field image of the bead. (b) Time-domain position of the trapped bead obtained with the image processing. The trapping laser power was 51 mW.