Figure 5.

Model calibration with dose-dependent target gene expression data from normal and perturbed conditions. Primary mouse hepatocytes were pre-treated with Ruxolitinib or DMSO control for 1 h prior to hIL-6 stimulation, or left untreated. Dose-dependent analysis of target gene expression was performed by applying 0.1–500 ng/mL hIL-6. At indicated time points (1, 6, and 24 h), target mRNAs were quantified by qPCR (A Socs3 and Cxcl10; B Fgg, Hamp, and Il33; C Fgg, Hamp, Il33, Apcs, Hp, and Hpx; D Socs3). Filled circles: log-transformed experimental data; solid lines: model trajectories. Dashed lines indicate the measurement noise as estimated by the error model. a.u., arbitrary units. For additional replicates used for model calibration see Appendix Figure S4.