FIG 1.

Caveola-related proteins are overexpressed in glioblastomas. (A) Analysis of the expression of caveolin-1 mRNA in a panel of GSCs, U251 glioma cells, and human umbilical vein endothelial cells was performed by reverse transcription-PCR (RT-PCR). In brief, cDNA was generated and subjected to RT-PCR, as described previously (3). GAPDH mRNA levels of expression were used as a control. The primers used for this project are listed in Table S2 in the supplemental material. NT, no template. (B) mRNA levels of caveolin-1, PTRF, and CD36 in normal tissue and glioblastomas, analyzed using the publicly available TCGA_GBM data set with the HG-U133A platform (TCGA Research Network, http://gliovis.bioinfo.cnio.es). P values were calculated using two-tailed Student's t test. (C) Protein levels of caveolin-1, PTRF, and CD36 analyzed by Western blotting of normal brain tissue (NB) and surgical human glioblastoma (GB). Tubulin expression was used as a loading control.