FIG 2.

The LBC binds to Fab-7 GAGA3 and -4, roX1, and roX2. Probe Fab-7 GAGA3+4 (A and B), roX1 (C), or roX2 (D) was incubated with 6- to 22-h nuclear extracts (NE) with or without (−) either a control preimmune rat or rabbit serum, a rat polyclonal E(y)2 antibody, two different rabbit polyclonal CLAMP antibodies (A and B), rabbit polyclonal GAF antibody, or a rabbit polyclonal Mod(mdg4) antibody. (A and B) The nuclear extract, antibody, and labeled probe were added together, and the reaction mixture was then incubated for 30 min prior to gel electrophoresis. (C and D) The nuclear extract was first preincubated with the indicated antibody for 30 min; labeled probe was then added, and the reaction mixture was incubated for an additional 30 min prior to gel electrophoresis.