FIG 6.

Characterization of primary CD4⁺ α₄β₇⁺ T cells. Human CD4⁺ cells were enriched from PBMCs of two separate donors and cultured in retinoic acid for 9 days prior to analysis. (A) Expression of CD4, β₇ integrin, CD45RO, and CCR5 measured by flow cytometry. (B) Binding of MAdCAM-1–Fc to cells from both donors. Binding was measured without inhibitors, with the CD4-blocking antibody leu3a, or with a combination of leu3a and 2B4 antibodies.