FIG 1.

Release of influenza virus proteins from HANAM1M2 and MAYNAM1M2 transfected 293T cells. (A) SDS-PAGE under reducing conditions followed by Western blotting of lysates and supernatants from 293T cells transfected with plasmids containing HA (A/Aichi/2/68), NA (A/Singapore/1/57), M1 (A/Hong Kong/8/68), and M2 (synthetic) and harvested at 48 h posttransfection. (B) Bar plot showing the averaged (n = 4) and lysate-normalized relative intensities of the Western blot bands. Relative M1 release levels into the supernatant were similar in HANAM1M2 and MAYNAM1M2 transfected cells. NA release to the supernatant was increased in MAYNAM1M2 transfected cells.