TABLE 2.
Summary of glycoprotein spacing measured by power spectrum analysis and line plot
| Particle type | Top-view measurement (PS)a |
Side-view measurement (avg ± SD [nm])b | ||
|---|---|---|---|---|
| Peak (nm) | μ fit (1/nm) | Sigma fit (1/nm) | ||
| Spherical with HA | ||||
| Without M1 | 9.46 | 0.11 | 0.02 | 8.3 ± 1.4 |
| With M1 | 8.90 | 0.11 | 0.03 | 8.8 ± 1.2 |
| Filamentous with HA | 8.36 | 0.12 | 0.02 | 8.2 ± 1.1 |
| Spherical with MAY | ||||
| Without M1 | 9.17 | 0.11 | 0.02 | 8.0 ± 1.0 |
| With M1 | 8.73 | 0.11 | 0.03 | 8.5 ± 1.4 |
| Filamentous with MAY | 8.18 | 0.12 | 0.02 | 8.2 ± 0.8 |
a
Values were obtained by power spectrum analysis from transverse slices of glycoproteins on the tops of the particles. Mean spatial frequency and sigma values were obtained from modified two-term Lorentzian-Gaussian fit of the power spectrum for filamentous particles and for spheroidal particles with and without an M1 layer containing either HA or MAY. Glycoprotein spacing at the peak of the curve is shown.
b
Mean and standard deviation (n = 20) of glycoprotein spacing obtained from density line scans on horizontal slices of the glycoprotein spikes.