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The loss of VCP prevents Gn from colocalizing with the Golgi complex. Huh7 cells were treated with either negative-control siRNA (A) or siRNA targeting VCP (B), followed by infection with MP12 at an MOI of 0.1. Cells were fixed, permeabilized, and stained for Gn, trans-Golgi network protein 46 (TGN46), and DAPI at 24 h postinfection. Arrowheads indicate areas of TGN46 and Gn colocalization (A) or areas that lack TGN46 and Gn colocalization (B).
