FIG 3.

CD2v deletion attenuates BA71. Pigs were immunized with 10³ PFU of either BA71 (discontinuous lines) or BA71ΔCD2 (continuous lines) and different parameters were monitored, including fever, viremia, death, and induction of specific antibodies and T cells. (A) Rectal temperatures were taken for individual pigs at different days after infection with either BA71 (discontinuous line) or BA71ΔCD2 (continuous line). (B) Average viremia kinetics from pigs immunized with BA71 (gray bars) or BA71ΔCD2 (white bars) are represented together with their corresponding standard deviations (right y axis). Virus titers are represented in the logarithmic scale as genome-equivalent copies per milliliter of serum. The percentage of surviving pigs after infection with BA71 (discontinuous line) or BA71ΔCD2 (continuous line) is also represented (left y axis). (C) The induction of ASFV-specific antibodies was measured by ELISA, and data corresponding to the kinetics of antibody induction for the 12 individual pigs inoculated with BA71ΔCD2 (continuous lines) are shown as optical density values. Averages and standard deviations for 6 pigs infected with BA71 are also represented. (D) Data correspond to the number of ASFV-specific T cells detectable in blood of the 12 pigs inoculated with BA71ΔCD2 24 days after its administration. Values correspond to the number of T cells that specifically secrete IFN-γ after BA71ΔCD2 in vitro stimulation per million peripheral blood mononuclear cells (PBMCs). *, ≥300 IFN-γ-secreting cells.