FIG 3.

HCV infection induces SMAD1/5 phosphorylation and ID1 expression. (A and B) P-SMAD1/5 (S463/S465) (A) and ID1 (B) expression status in Huh7.5 cells and HCV 2a (JFH1)-infected Huh7.5 cells. The actin expression status was determined and included as an internal control for comparison of protein expression. Densitometric scanning of Western blot results is shown on the right. Values are means from three independent experiments ± SD. Statistical significance was analyzed using the two-tailed Student t test: *, P < 0.05; **P < 0.01. (C and D) qRT-PCR data from nine liver biopsy RNAs for endoglin (C) and ID1 (D). Results from control liver RNAs (n = 2) are shown for comparison.