FIG 5.

HCV infection alters the ID1 downstream antiapoptosis Bcl-2 protein and enhances cell proliferation and CSC marker proteins. (A and B) Infection of Huh7.5 cells with cell culture-grown HCV genotype 2a (clone JFH1) enhanced Bcl2 (A) and CSC marker proteins (N-cadherin, Notch1, Nanog, and Sox2) (B) as determined by Western blotting. (C) Cell cycle progression markers (cyclin E and cyclin D1) and a Notch signaling marker (Hes1) were examined similarly from infected cells by Western blotting. Densitometric scanning for comparison of protein status is shown on the right. Statistical significance was analyzed using the two-tailed Student t test: *, P < 0.05; **P < 0.01.