TABLE 1.
Primers utilized to introduce mutations in the infectious clone of ZIKV isolate GZ01
| Primer | Sequence (5′-3′)a | Application |
|---|---|---|
| SLA-TL-M-F | CAGACTGCGAgtcTTCGAGTTTGAAGCG | To introduce mutations into SLA top loop |
| SLA-TL-M-R | ATTCACACAGATCAACAAC | |
| SLA-S2-M1-F | AAGCTAGCAAgacTATCAACAGGTTTTATTTTG | To introduce mutations to disrupt the stem 2 of SLA |
| SLA-S2-M1-R | TCGCTTCAAACTCGAACTG | |
| SLA-S2-M2-F | AGTTGTTGATgtcTGTGAATCAGACTG | To introduce mutations to restore the stem 2 of SLAb |
| SLA-S2-M2-R | CTATAGTGTCACCTAAATGC | |
| 5′-CS-M-F | AAGCTAGCAAcattgTATCAACAGG | To introduce mutations into 5′ cyclization sequence |
| 5′-CS-M-R | TCGCTTCAAACTCGAACTG | |
| 3′-CS-M-F | ACGCAAAACAcaatgTTGACGCTGGGAAAGACCAG | To introduce mutations into 3′ cyclization sequence |
| 3′-CS-M-R | TTTCCGGGGGGTCTCCTC | |
| GAA-F | AGTCAGTGGAgcagcaTGCGTTGTGAAGC | To introduce mutations into the GDD motif of NS5 |
| GAA-R | GCCATTCGTTTGAGCCTA |
a
Mutations are indicated in boldface letters.
b
This mutant was achieved based on pregenerated SLA-S2-M1 mutant.