FIG 1.

Identification of ZIC2 as a ubiquitinated target of K-Rta. (A) Schematic illustrating the identification of ubiquitinated target proteins of K-Rta using the TREx-K-Rta His-ubiquitin (Ub) 293 cell line. His-ubiquitin-conjugated proteins were enriched, followed by MS analysis. (B) Whole-cell lysates of TREx-K-Rta 293 cells and TREx-K-Rta His-ubiquitin 293 cells were subjected to nickel affinity chromatography and analyzed by immunoblotting with polyhistidine antibody. (C) Spectral count of ZIC2 protein identified in Ni-NTA pulldowns from untreated and Dox-treated TREx-K-Rta His-ubiquitin 293 cells. Rep, repeat assay.