FIG 6.

ZIC2 maintains H3K27me3 marks at the genomic region harboring bivalent histone marks. (A) Positions of qPCR primer pairs (specific for regions A to I) on the K-Rta promoter used for ChIP assays. (B) BCBL-1 cells were infected with lentivirus expressing shScrm or shZIC2 for 72 h. ChIP-qPCR analyses of the selected regions were performed using antibodies against H3, H3K4me3, AcH3, and H3K27me3. (C) iSLK.219 cells were infected with lentivirus expressing shScrm or shZIC2 for 72 h. The localization of H3, H3K4me3, AcH3, and H3K27me3 on the K-Rta promoter was detected by ChIP-qPCR assays. Normal rabbit IgG was used as a negative control. The data are presented as means ± SDs. *, P < 0.05; **, P < 0.01.