TABLE 2.
List of primers
| Primer application and gene or region | Sequence |
|
|---|---|---|
| Forward | Reverse | |
| RT-qPCR primers | ||
| ZIC2 | GTCCACACCTCCGATAAGCC | CTCATGGACCTTCATGTGCT |
| GAPDH | TCGCTCTCTGCTCCTCCTGTTC | CGCCCAATACGACCAAATCC |
| ChIP-qPCR primers | ||
| Peak 1/ORF8 | ATCAACACCACGCTAAACGC | TGGGCTGCCAGACCAAATA |
| Peak 2/ORF9 | ATAACCCTGTCATCCAGTCCG | CCAGCACGAAGCGCCTAAT |
| Peak 3 | TACCAGCAGTGAACCGACCAG | CGCCCTTCAGTGAGACTTCGT |
| Peak 4 | TTTCTTCCTCGACAGGTCTTC | ATATCACTGCGGCCCAACT |
| Peak 5/K6 | TGTGGGCTTTGAGTTCTGTC | GGGTCGTGGGAATATGGTAC |
| Peak 6 | CAGGAGACGGCATTTGACC | TTCCCGAGTTGACCCAGTA |
| Peak 7 | CACTGCCGCATTATTGTCTG | GCTCCATCGCCTCTTTCGT |
| Peak 8 | CCTAACCCACGTCACATCAC | CAGGTAACAAAGCCGCAGA |
| Peak 9/ORF45 | GTGCGTTTATGAGCGGAGTT | ACTTGGCTGGGATTGGTTC |
| Peak 10 | CCGCTAACGAGGTACAGGA | GACGCCCTTGCGAACACTT |
| Peak 11 | CGTAACCCAGCAGTGACCT | TTGGATAGACGGCTTGGAG |
| Peak 12/K9 | CAGCGGTGTCTGTGGGATT | GGAAGGCTGGTGGTTTGGT |
| Peak 13 | CAGGAAACATAGAAGTTGCGGA | TCTTGTCACTCTGTCCGGGTA |
| Peak 14/K13 | GTTGCGTTGGTTGGGAGTT | CGATCTGAGCCATTGAAGC |
| Peak 15/LANA | ATAAGTCAGCCGGACCAAGC | GGGCACCAATCAGAAAGTAGC |
| ORF22 | CAGCAGTGGTGTTCCCTCT | GTCGCCTTCTGTGGCTCTA |
| ORF64 | GACCCGCTACGACCATTCT | GAACTGTGATCCCGCCTCTT |
| ORF50 promoter_A | TGTTTCCACGGTAATGTCG | TTTGCGGTCCACTCTATCC |
| ORF50 promoter_B | GGCGACGTATGCGTGAGAT | CACCGAACCAGGCAACACA |
| ORF50 promoter_C | TATTTCCCACGACATACTTCC | TCCGACGATGAGTATGATTGA |
| ORF50 promoter_D | GCAAGCAAGCTGGTGTTCT | TTGGATACCCTGGTGATGC |
| ORF50 promoter_E | GCATGACTCCAACCGTCTC | GGCGGGAGCTTAGCTTGAT |
| ORF50 promoter_F | CTGACCCACCAAGGAGAAC | GTGCCACCAATGTATGACC |
| ORF50 promoter_G | AGCCAGCGTATGCTTCAGG | CCACATCGGGTTTCGTCAA |
| ORF50 promoter_H | GCTACCGGCGACTCATTAA | TGCCTGGACAGTATTCTCACA |
| ORF50 promoter_I | CGGGTGTCAGGGCTCTTAT | CTGAAAAGCACCGCCCGAT |