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. 2017 Oct 13;91(21):e00923-17. doi: 10.1128/JVI.00923-17

FIG 1.

FIG 1

Plasma Ab binding and neutralization profiles of DNA/rAd5-vaccinated RMs. (A) DNA/rAd5 vaccinations in 4 neonatal RMs and 4 adult RMs. RMs were immunized with DNA prime 3 times, with an rAd5 boost. PBMCs and plasma were collected on the vaccination day and at 2 weeks after each vaccination, with sampling time points indicated on the vaccination timeline; vaccine-induced Ab repertoires of blood memory B cells were studied 4 weeks after rAd5 boost. (B) Plasma Ab binding (AUC, area under the curve) to VRC-A, VRC-B (B.HxB2/BaL), and VRC-C (C.97ZA012) HIV-1 gp120 proteins and B.MN gp41 proteins in 4 neonatal (red) and 4 adult (blue) RMs at 4 weeks after rAd5 boost using the BAMA assay. Each symbol represents one animal. (C) Plasma antibody binding (mean) kinetics in DNA/rAd5-vaccinated RMs at 2 weeks after each DNA prime and 4 weeks after rAd5 boost using ELISA. The arrow represents the time of immunization. (D) Plasma samples from DNA/rAd5-vaccinated adult and neonatal RMs were screened for a panel of autologous and heterologous virus isolates via TZM-bl assay. Murine leukemia virus (MuLV) was used as control. The neutralization key is shown at the bottom.