FIG 3.

Flow cytometric staining of blood memory B cells using fluorophore-labeled CON-S and VRC-A gp120 Envs compared with fluorophore-labeled gp140 Envs. (A) Flow plots of gp140 and gp120 (CON-S or VRC-A) staining of Env-reactive memory B cells in 4 neonatal RMs and 4 adult RMs. Memory B cells which bound both BV421-labeled Envs (x axis) and AF647-labeled Envs (y axis) were defined as double positive Env-reactive memory B cells. (B) Table showing the frequencies of double positive Env-reactive memory B cells in each RM and the mean frequencies of the 8 RMs. For statistics, theWilcoxon signed-rank test was used.