TABLE 2.
Polyreactivities of vaccine-induced gp41-reactive and gp120-reactive MAbs from DNA/rAd5-vaccinated RMsa
| MAb ID | Specificity | Anaerobe WCL reactivity | Aerobe WCL reactivity | Autoantigen binding by ELISA | HEp-2 cell reactivity |
|---|---|---|---|---|---|
| DH782 | gp41 | − | − | RNP | − |
| DH783 | gp41 | − | − | − | − |
| DH785 | gp41 | + | + | RNP | − |
| DH786 | gp41 | − | − | − | − |
| DH787 | gp41 | + | + | − | − |
| DH788 | gp41 | − | − | − | − |
| DH789 | gp41 | − | − | − | − |
| DH790 | gp41 | − | − | Histone | − |
| DH791 | gp41 | − | − | − | − |
| DH792 | gp41 | + | + | − | − |
| DH793 | gp41 | − | − | − | − |
| DH794 | gp41 | + | − | − | Cytoskeletal, perinuclear coil, vimentin |
| DH795.1 | gp41 | + | + | − | − |
| DH795.2 | gp41 | + | + | − | − |
| DH796 | gp120 | − | − | Histone | − |
| DH797 | gp120 | − | − | − | − |
| DH798 | gp120 | + | − | − | − |
| DH799 | gp120 | − | − | − | − |
| DH800 | gp120 | − | − | − | − |
| DH801 | gp120 | + | + | − | − |
| DH802 | gp120 | + | + | SSA, SSB, Scl-70, Sm, RNP | Nuclear +, cytoplasmic +, surface + (rim), nucleoli − |
| DH803 | gp120 | − | − | − | − |
| DH804 | gp120 | − | − | − | − |
| DH805 | gp120 | + | + | − | − |
a
MAbs were assayed for cross-reactivity with anaerobic and aerobic bacterial WCLs of autologous macaque fecal samples, host antigens (autoantigens binding by ELISA), and HEp-2 cells (indirect fluorescence antibody assay [IFA]; Zeus Scientific).