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Effect of deleting genes encoding VTPs on the ability of CyHV-3 FL BAC recombinant plasmids to reconstitute infectious virus. CCB cells were transfected with the plasmids indicated. At 2 and 4 dpi, the cells were examined by epifluorescence microscopy (the BAC cassette encodes EGFP). Scale bar, 1 mm. Plasmids able or not able to reconstitute infectious virus are labeled in green or red, respectively.
