FIG 4.

Testing plasmids recombinant for CyHV-3 ORFs predicted to encode essential VTPs. For ORFs identified as essential in Fig. 3, additional recombinant plasmids (ORFX Rev1 and ORFX NS) were produced (see Fig. 2, description in the rectangular frame) and tested for their ability to reconstitute infectious virus after transfection into CCB permissive cells. At 6 days posttransfection, the cells were examined by epifluorescence microscopy for detection of EGFP. Scale bar, 1.5 mm.