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. 2017 Oct 13;91(21):e01209-17. doi: 10.1128/JVI.01209-17

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Copyright © 2017 Vancsok et al.

This is an open-access article distributed under the terms of the Creative Commons Attribution 4.0 International license.

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FIG 5 — Effects of deleting nonessential VTPs on CyHV-3 growth in vitro. (A) Multistep growth curves. CCB cells were infected with the viruses indicated and the titer (PFU/ml) in the cell supernatant was determined at the indicated time points postinfection. Data presented are the means ± the standard errors of the mean (SEM) of triplicate measurements. Significant differences between the deleted viruses and the TK BAC virus were tested by using two-way ANOVA, taking genotype and time postinfection as variables. (B) Plaque size assay. CCB cells were infected with the viruses indicated, and plaque areas were measured over time. The data presented are means ± the SEM for the measurement of 25 randomly selected plaques. Two-way ANOVA was used to test the significance of the results, taking genotype, time postinfection, and interaction between genotype and time postinfection as variables (*, P < 0.05; **, P < 0.01; ***, P < 0.001).