FIG 7.
PCR detection and characterization of CyHV-3 genomes recovered from infected dead carp. DNA was extracted from the gills of three dead carp from each of the groups infected with the recombinant viruses deleted for a nonessential gene (TK BAC ΔORFXgalK) during the course of the experiment described in the legend of Fig. 6. In the TK BAC Δ25galK infections, no fish died, and hence three fish were selected randomly from among the living fish at 30 dpi. A carp infected with TK BAC virus and a mock-infected carp were used as positive and negative PCR controls, respectively. PCRs were performed with the appropriate ORFXintfw/ORFXintrev primer pair and with the ORF112intfw/ORF112intrev primer pair. The images are photographs of agarose gels. The numbers on the left of each panel are marker sizes (bp). NC, negative control.