FIG 9.

Effect of ORF25 deletion on viral tropism according to qPCR analysis. At time zero, carp (average weight, 6.10 g ± 1.39 g; average age, 6 months old) were infected for 2 h by immersion in water containing the virus indicated at 800 PFU/ml and then returned to larger tanks. At the indicated time points, fish were sampled and submitted to qPCR analysis. The data obtained for each fish (according to viral genotype and time postinfection) are represented by the same symbol to allow correlation of the data obtained for the two tested organs. The number of viral genome copies is expressed as the log₁₀ per 10⁶ carp glucokinase gene copies. Individual values represent the mean of duplicate measurements. Mock-infected fish were used as a negative control and no viral genome copies were detected in these fish. The number of positive fish among six analyzed fish is represented by gray bars. Viral charges [viral genome copies/10⁶ carp glucokinase gene copies (log₁₀)] were compared using one-way ANOVA taking the viral genotype as a variable.