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Comparison of the replication efficiency of a g-1 HEV clinical isolate in Huh7 and ORF4-Huh7 cells. qRT-PCR detection of the HEV sense-strand RNA level in Huh7 or ORF4-Huh7 cells infected with a g-1 HEV clinical isolate. Total RNA was isolated at different days postinfection, followed by estimation of HEV sense and GAPDH RNA levels. The ratios of HEV sense/GAPDH values are represented as means ± SEM.
